Mitochondrial SWATH Analysis

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Sequential Window Acquisition of all Theoretical fragment ions (SWATH) is a new mass spectrometry acquisition mode technology. As a DIA (Data Independent Acquisition) technology, SWATH divides the whole scanning quality range of MS into several small windows and splits all the ions in each window in turn, so that all the peptide ions in the scanning range can be scanned by high-speed first-order MS and then analyzed by secondary MS/MS. Therefore, SWATH can quantitatively determine almost all detectable molecules in complex samples. SWATH combines the characteristics of high-throughput detection of shotgun proteomics and the advantages of accurate quantitative analysis and is a real panoramic mass spectrometry detection method MS/MS ALL.

Figure 1. Targeted data extraction for SWATH-MS-based protein quantification. ( Frederick K., Ciborowski P., 2016)

Creative Biogene adopts AB SCIEX Triple-TOF 5600 plus high-resolution mass spectrometry system, which has the ability of fast scanning speed and quantitative sensitivity of series quadrupole mass spectrometry system, combining the high resolution, accurate mass number stability, high sensitivity, and high-speed scanning characteristics of mass spectrometry system to provide SWATH quantitative proteome analysis service. You only need to send your sample to us, and we will be responsible for all the follow-up matters of the project, including protein extraction, protease digestion, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, bioinformatics analysis.

Service Flow

Creative Biogene will provide you with a detailed technical report when the project is delivered, including experimental steps, relevant MS parameters, peptide/protein identifications and intensities, MS images (high resolution), and detailed raw data. Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.

Technical characteristics

• Quantitative identification of all molecules in complex samples, including low abundance proteins and peptides.
• The quantitative accuracy is high (equivalent to that of MRM), but debugging the sample determination method in advance is not needed as MRM does.
• More than 2000 proteins can be quantitatively detected in one experiment.
• Complete and comprehensive information storage, which can store the data of each peak of MS, MS/ MS, and there is no need for repeated detection of the same sample.

Our Advantages

• Rich experience in mitochondrial quantitative proteomics research
• Outstanding research team
• Unique integrated service for mitochondrial research
• Reliable data and results
• Rapid turnaround and cost-effective

Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

Reference

1. Frederick K., Ciborowski P., “ 9 - SWATH-MS: Data Acquisition and Analysis. ” Proteomic Profiling and Analytical Chemistry (Second Edition), 2016, 161-173. (https://doi.org/10.1016/B978-0-444-63688-1.00009-4.)

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