Mitochondrial PCT & SWATH Analysis

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Pressure Cycling Technology (PCT) technology is a rapid crushing of trace samples based on cyclic ultra-high pressure. The cycle between ultra-high pressure and standard atmospheric pressure can induce the dissolution of matrix and protein, promote and accelerate protein hydrolysis, and be used for proteome sample preparation. It has the advantages of high speed, low loss, simple operation, and high fidelity. For clinical samples, PCT can also inactivate potentially contaminated infectious microorganisms and restore the original state of the samples to the maximum extent.

Creative Biogene has established a very efficient PCT-SWATH standardization process. The clinical large cohort samples can be transformed into big data by high-throughput PCT-SWATH technology. PCT-SWATH technology combines PCT and SWATH technology, which is especially suitable for batch analysis of proteome in large queues (tens of hundreds or even thousands of samples) and a small number of samples (such as tissue biopsy samples). It can analyze the mass protein data generated by mass spectrometry, compare with each other and data mining, to find and quantify the specific protein expression, and finally obtain some disease markers or explore the relevant molecular mechanism behind the occurrence of some diseases.

Work Flow

Creative Biogene will provide you with a detailed technical report when the project is delivered, including experimental steps, relevant MS parameters, peptide/protein identifications and intensities, MS images (high resolution), and detailed raw data. Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs, you just need to tell us your experimental purpose and send us your cells, and we will be responsible for all the follow-up matters of the project.

Technical characteristics

• Compatible with all chemical dissolution buffers and analytical methods.
• Grinding operation and shear force to avoid macromolecular damage.
• Accurate temperature control, including reactions at low or high temperatures, not only improves the extraction effect but also reduces the influence of chemical reagents and high temperature on the sample.
• Without contact with metal substances, chemical substances prone to redox reactions can be treated and preserved to prevent the activation of metalloproteinases.
• Instantaneous and uniform energy distribution.
• Precise control of biomolecule dissolution.
• Suitable for all commonly used organic solvents.

Our Advantages

• Rich experience in mitochondrial quantitative proteomics research
• Outstanding research team
• Unique integrated service for mitochondrial research
• Reliable data and results
• Rapid turnaround and cost-effective

Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

Reference

1. Guo T. N., et al. “ Rapid mass spectrometric conversion of tissue biopsy samples into permanent quantitative digital proteome maps. ” Nature Medicien, 2015, 21: 407-413.

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