MRM & SRM of Mitochondrial Proteomics

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Selected reaction monitoring (SRM), also known as Multiple Reaction Monitoring (MRM) is a targeted quantitative proteomics method for the study of target protein molecules. Based on the information of the target molecule, MRM/SRM selects the data to collect the mass spectrum data, collects the signal that conforms to the rules of the target ion, and removes the interference of the ion signal that does not conform to the rule.

Figure 1. Schematic diagram of the principle of MRM/SRM.

MRM/SRM mass spectrometry analysis goes through three stages. First, the parent ions which are consistent with the specificity of the target molecule are screened by MS. Secondly, these parent ions are broken by collision to remove the interference of other ions. Finally, only the selected specific MS/MS2 ions are collected by mass spectrometry. MRM/SRM mass spectrometry is a highly accurate technique for quantitative identification of proteins, and it is an excellent method for a one-time accurate quantitative study of multiple target proteins in complex samples. If the target peptide labeled by isotope is used as the internal reference, the absolute quantitative identification of protein can be achieved.

MRM/SRM monitors each precursor ion/product ion transition at a time, while PRM analyzes all productions derived from a precursor ion in concert with high resolution and mass accuracy. Both MRM/SRM and PRM allow relative and absolute quantification of proteins, peptides, and metabolites.

After receiving the information on the target protein from our customers, we can provide a one-stop MRM/SRM quantitative protein analysis service. It includes the establishment and optimization of the MRM method, selection of specific peptides, isotope-labeled peptides, mass spectrometry analysis, original data analysis, bioinformatics analysis.

Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.

Personalized MRM/SRM Bioinformatics Analysis Service

• MRM/SRM spectrum analysis, protein data quality evaluation.
• PCA analysis of multi-component samples.
• Identified protein function annotation: GO function annotation, KEGG function annotation, and COG function annotation.
• Statistical analysis of differentially expressed proteins: Wayne diagram, Volcano Plot.
• Cluster analysis of differentially expressed proteins: hierarchical clustering, K-means clustering.
• Differential protein interaction Analysis: protein interaction Network Analysis based on STRING Database.

Technical characteristics

• The sensitivity is high, the ions consistent with the target ions are selected by two-stage mass spectrometry, the interfering ions are eliminated, the signal-to-noise ratio is greatly improved, and the accuracy of detecting target ions is improved.
• High throughput, up to 200 proteins can be identified at one time.
• It can be used for absolute quantitative analysis without relying on antibodies.
• High precision identification of low abundance protein, the quantitative range spans 4 orders of magnitude

Our Advantages

• Rich experience in mitochondrial targeted proteomics research
• Outstanding research team
• Unique integrated service for mitochondrial research
• Reliable data and results
• Rapid turnaround and cost-effective

Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

Reference

1. Chen M.X., et al. “ Absolute quantification of proteins in rice leaf based on multiple reaction monitoring mass spectrometry. ” Chinese Journal Rice Science, 2020, 34(3): 278-286.

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