| Description |
This product is a kit specially used for the purification of plant cell mitochondria. It has the following characteristics:n1. Ready-to-use kits, users do not need to prepare various solutions separately.n2. Provide two operation schemes: rough extraction and fine extraction. The rough extraction uses the differential separation principle of a conventional bench-top refrigerated centrifuge for mitochondrial rough extraction. The resulting mitochondria contain a small amount of contamination of other organelles and can be used for subsequent SDS-PAGE, Western, ELSIA And proteomic analysis can also be used for PCR-level mitochondrial DNA and RNA extraction.n3. Fine extraction uses a density gradient prepared by frozen ultracentrifugation (centrifugal force up to 40,000g) to further separate the crude mitochondria and other cell components to obtain mitochondrial sperm extract, which is suitable for subsequent coupling analysis of in vitro mitochondrial protein synthesis and mitochondria Fine experiments such as component positioning. It can also be used for subsequent SDS-PAGE, Western, ELSIA, proteomic analysis and sequencing-level mitochondrial DNA and RNA extraction. |
