MtDNA Mutation Screening

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A cell contains many mitochondria, each having dozens of copies of the mitochondrial genome. The mutation rate of the mitochondrial genome is higher than that of the nuclear genome, which leads to heterogeneous populations of mitochondrial DNA in the same cell or even in the same mitochondria. When a cell divides, its mitochondria divide into two daughter cells. However, the separation process of mitochondria is random, and its tissue structure is much worse than the high-precision nuclear chromosome separation process during mitosis. The result is that the two daughter cells receive similar but not identical copies of mitochondrial DNA.

Compared with nuclear DNA, mitochondrial DNA is more vulnerable even with a repair mechanism since the repair capacity is limited. Most mitochondrial proteins are encoded by nDNA, including proteins that regulate mitochondria, mitosis, fusion, division and signal transduction. This nuclear genome mutation can lead to mitochondrial genome instability, including a large number of mitochondrial DNA deletions and point mutations.

Creative Biogene provides an integrated platform for when mitochondrial diseases may be caused by mutations and polymorphisms of the mitochondrial genome. We can provide mtDNA mutation screening and a full range of follow up and analysis services for our worldwide customers.

Service Content

• High-throughput sequencing and basic Analysis
• Mutation verification to expand the MtDNA mutation spectrum
• Analysis of interaction between nuclear DNA and mtDNA under MtDNA mutation
• Genetic analysis and summary
• Target gene sequence query, specific primer design, synthesis and optimization
• Assist in the diagnosis of related diseases (not as a basis for clinical diagnosis, but only for scientific research)
• Customized personalized service content

Service Flow

Our Advantages

• Rich experience in the study of mitochondrial DNA mutation
• Outstanding research team
• Unique integrated service for mitochondrial research
• Reliable data and results
• Rapid turnaround and cost-effective

Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

References

1. Chial H., et al. (2008). “ MtDNA and mitochondrial diseases.” Nature Education. 1(1): 217.
2. Ryzhkova A.I., et al. (2018). “ Mitochondrial diseases caused by mtDNA mutations: a mini-review. ” Ther Clin Risk Manag. 14: 1933-1942.

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