| Description |
Superoxide anion radicals, as a kind of free radical generated during the metabolism of living organisms, can attack biological macromolecules, such as lipids, proteins, nucleic acids and polyunsaturated fatty acids, to cross-link or break them, causing damage to cell structure and function, and are very closely related to the aging and pathology of the organism. The AP-TEMED system produces superoxide anion, which reacts with hydroxylamine hydrochloride to form NO2-, and NO2- interacts with sulfanilic acid and α-naphthylamine to produce a red azo compound with a characteristic absorption peak at 530 nm, and the scavenging ability of the sample for superoxide anion is negatively correlated with the absorbance value at 530 nm. |
