| Product Name |
mtCheck™ Aconitase Activity Assay Kit (Microplate) (KITA1053) |
| Description |
Aconitase(ACO), an enzyme in the tricarboxylic acid cycle, catalyzes the conversion of citric acid to isocitrate. Citric acid itself is not easy to oxidize. Under the action of aconitase, the hydroxyl group is transferred from the β carbon atom to the α carbon atom through dehydration and water addition to generate isocitric acid which is easy to deoxidize, which is a further oxidative decarboxylation reaction. Make preparations. ACO catalyzes the conversion of citric acid to isocitric acid, and the oxidative decarboxylation of isocitric acid reduces NAD+ to NADH, resulting in an increase in light absorption at 340nm. |
| Catalog Number |
KITA1053 |
| Suitable Sample |
Cell,Tissue,Serum |
| Species Reactivity |
All |
| Size |
96 Tests |
| Storage Instruction |
Store components of the kit at 4°C or -20°C |
| Usage Statement |
Our products are intended for research use only and are not to be used for other purposes, including but are not limited to in vitro diagnostic uses, ex vivo or in vivo therapeutic uses. |
| Detection Method |
Absorbance microplate reader |
