Mitochondrial Protein Molecular Weight Determination

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Molecular weight is the basic physical and chemical property parameter of organic compounds. Molecular weight is also the most important parameter when identifying peptides and proteins and a critical piece of data for the approval of genetic engineering products. The easiest way to identify the size and purity of a protein is by SDS-PAGE, determined by SDS-PAGE.

MALDI TOF MS is a new type of soft ionization biological mass spectrometry developed in recent years, which has the characteristics of low sample consumption, high sensitivity, fast determination speed, easy to achieve high throughput, large-scale identification of proteins, and determination of the molecular weight of biological macromolecules, which provides a powerful analysis and testing method for protein research.

Figure 1. MALDI TOF/TOF mass spectrometry system. ( Antony C.,et al., 2012)

Creative Biogene uses Bruker's ultrafleXtreme™ MALDI TOF/TOF mass spectrometry system, with an accuracy of 0.1% mi 0.01%, which is much higher than the conventional SDS-PAGE and high-performance gel chromatography techniques. At present, the molecular weight range of biological macromolecules can be determined from 700 to 600000 Da. It is simple and intuitive and can be used to determine the mixture of macromolecules directly, and there is almost no fragment peak. Creative Biogene provides biomolecule determination services to analyze the molecular weight of target mitochondrial proteins or peptides, detect the presence of target proteins or peptides and protein dimers, and evaluate the purity of samples.

Work Flow

Creative Biogene will provide you with the experimental steps, relevant MS parameters, high-resolution MS images, raw data, and protein molecular weight, and purity analysis results immediately after the end of the experiment.Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.

h3>Our Advantages

• Rich experience in mitochondrial protein molecular weight determination
• Outstanding research team
• Unique integrated service for mitochondrial research
• Reliable data and results
• Rapid turnaround and cost-effective

Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

Reference

1. Antony C., et al. “ Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. ” FEMS Microbiology Reviews, 2012, 36(2): 380-407.
2. Signor, L., Boeri E.E. “ Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) mass spectrometric analysis of intact proteins larger than 100 kDa.” J. Vis. Exp., 2013, 79: e50635.

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