Mitochondrial Coenzyme Determination
Creative Biogene uses biochemical and HPLC methods to detect coenzyme substances, such as lactate dehydrogenase, acetaldehyde dehydrogenase, citric acid synthase, and pyruvate decarboxylase. We can detect coenzyme activity efficiently and accurately.
The coenzyme is a small organic non-protein molecule whose use is to carry chemical bases within enzymes. Many coenzymes are phosphate soluble vitamins. But non-vitamin substances may also be coenzyme, such as ATP-phosphate biochemical vehicles. Within cells, the coenzyme after reaction can be regenerated to maintain its intracellular concentration at a stable level. Because coenzyme regeneration is necessary to maintain the stability of the thiamine system of the enzyme reaction, coenzyme regeneration system has been widely used in laboratory research.
General Strategy
Creative Biogene uses biochemical methods to detect coenzyme substances, which can detect coenzyme substance activity efficiently and accurately. Currently, available coenzyme substances include NADH, NADPH, lactate dehydrogenase, acetaldehyde dehydrogenase, citric acid synthase, and pyruvate decarboxylase. Additionally, the Creative Biogene platform provides an HPLC method for determining coenzyme substances to meet diverse needs worldwide.
In addition to the following items, Creative Biogene also provides G6PDH, NAD-MDH/NADP-MDH, TrxR, PDC, ICDHc, NADPase, NAD-ME / NADP-ME, CK, FAS activity detection and NADH, NADPH content detection, and other services.
Lactate dehydrogenase (LDH) can catalyze lactic acid to pyruvate and NADH, NADH has a maximum absorption peak at 340nm, Creative Biogene uses colorimetry to detect LDH activity.
The oxidation of NADH to NAD, NADH catalyzed by NADH oxidase (NOX) is coupled with the reduction of DCPIP to reduce blue DCPIP to colorless DCPIP. The blue DCPIP has a maximum absorption peak at 600nm. Our scientists can calculate the reduction rate of blue DCPIP to detect the activity of NOX.
In the presence of coenzyme I, acetaldehyde dehydrogenase (ALDH) can catalyze acetaldehyde and NAD+ to form acetic acid and NADH, NADH has a maximum absorption peak at 340nm. Our scientists can detect the activity of ALDH by calculating the formation rate of NADH.
Citrate synthase (CS) catalyzes acetyl-CoA and oxaloacetic acid and further hydrolyzes to citric acid. The reaction promotes the conversion of colorless DTNB to yellow TNB and has a maximum absorption peak at 412nm. Creative Biogene uses spectrophotometry to detect CS activity.
Pyruvate decarboxylase (PDC) uses pyruvate as a substrate to catalyze the oxidation of NADH to NAD+. NADH has a maximum absorption peak at 340nm. Creative Biogene detected the activity of PDC by calculating the reduction of NADH.
Alcohol dehydrogenase (ADH) catalyzes NADH to reduce acetaldehyde to ethanol and NAD+, NADH has a maximum absorption peak at 340nm, Our scientists calculate the reduction of NADH to detect ADH activity.
Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.
Our Advantages
- Rich experience in determination and analysis of mitochondrial coenzyme
- Outstanding research team
- Unique integrated service for mitochondrial research
- Reliable data and results
- Rapid turnaround and cost-effective
Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.
For Research Use Only. Not For Clinical Use Or Individual Users.
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