Isolation and Extraction of Brain-Derived Mitochondria

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Mitochondria play an important role in the pathophysiology of many neurological diseases, and some neurodegenerative diseases are closely related to synaptic damage and synaptic mitochondrial dysfunction.

However, the evaluation of mitochondrial dysfunction and the efficacy of mitochondrial-targeted therapy in experimental models of neurodegenerative diseases and central nervous system injuries are limited by mitochondrial separation techniques. At present, density gradient ultracentrifugation (UC) is the only technique that can separate synaptic and non-synaptic mitochondrial subsets, but the yield of mitochondria prepared by this technique is low.

(−) technique does not have the capability or is a disadvantage. (+) technique has the capability, can perform well, or is an advantage. (++) technique has superior capability or has greater advantage compared to other methods. ( Hubbard, W.B., et al.,2019)

To solve this limitation, Creative Biogene uses the fractionated mitochondrial magnetic separation (FMMS) method to separate functional synaptic and non-synaptic mitochondria from the mouse cortex and hippocampus without the use of UC. Compared with UC, the synaptic mitochondrial protein production produced by the FMMS method is 3 times higher than that of UC.

A common problem with UC is that it is unable to obtain sufficient synaptic mitochondrial production from a low initial tissue volume for technical analysis. FMMS method can increase the production of brain-derived mitochondria be used for mitochondrial assessment, and can better detect mitochondrial damage in central nervous system injury and neurodegenerative diseases.

FMMS General Strategy

Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.

Our Advantages

• Rich experience in isolation and extraction of brain-derived mitochondria
• Outstanding research team
• Unique integrated service for mitochondrial research
• Reliable data and results
• Rapid turnaround and cost-effective

Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

References

1. Hubbard W.B., et al. “ Fractionated mitochondrial magnetic separation for isolation of synaptic mitochondria from brain tissue. ” Scientific Reports, 2019, 9: 9656.
2. Afanasyeva M.A., et al. “ Isolation of large amounts of highly pure mitochondria for “omics” studies. ” Biochemistry Moscow, 2018, 83: 76-85.

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