Mitochondrial GST Pull Down
Creative Biogene provides efficient and accurate integrated solutions for mitochondrial critical gene and protein verification, and a groundbreaking combination of biochips and data analysis platforms to help you easily and quickly identify important annotated genes and signal pathways.
Protein-protein interaction is one of the most important aspects of verifying key genes and proteins and the study of protein structure and function. GST pull-down fused the target protein with GST and immobilized the protein on the glutathione-affinity resin to support affinity with the target protein, acting as a "bait protein". The target protein solution passed through the column to capture the "capture protein (target protein)" that interacted with it. After elution, the conjugate was eluted and analyzed by electrophoresis to confirm the interaction between the two proteins or screen the corresponding target protein. Creative Biogene now provides GST pull-down detection technical services, the use of specific secondary antibodies for detection, can effectively avoid the signal interference of antibody heavy chain to the results, and significantly improve the detection effect.
Creative Biogene is a leading mitochondrial technology service provider with many years of experience in protein interaction analysis based on key genes and protein verification. Creative Biogene provides an efficient strategy for protein expression and purification, as well as an optimization scheme for pull-down-protein interaction analysis. We feature strict quality control and a short testing cycle to save your valuable time and budget, provide customizable one-stop service, and cover every step of your project from gene screening, synthesis, and detection to data reporting.
Service flow
Our technological advantages
- We offer one-stop service from preparing the GST fusion protein to analyzing protein interaction.
- Multiple groups of control experiments are performed to fully eliminate interference factors and contribute to a more accurate judgment of results.
- The experiment is repeated twice to eliminate the influence of random factors and make the result more reliable.
- Complete testing equipment, such as liquid mass spectrometry and Fortebio, is furnished as a companion to further analyze the testing results.
- The sensitivity of the silver staining technique is 100 times high than of coomassie brilliant blue (CBB) and the SDS-PAGE electrophoresis results of that are more distinct.
Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.
For Research Use Only. Not For Clinical Use Or Individual Users.
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