Mitochondrial Glycolysis Determination
Creative Biogene's platform uses the Seahorse XF analyzer to measure external proton flux and OCR oxygen consumption rate (OCR). It can be used to detect metabolic function in real-time. Glycolysis rate assay uses OCR and ECAR to determine mitochondrial acidification, thereby glycolysis proton release rate and biological energy metabolism spectrum of live cells help understand the early stage of metabolism transformation.
Glycolysis is a process of decomposing glucose into components used for biosynthesis or energy production, an important metabolic pathway for eukaryotic cells. Glycolysis plays an important role in a series of physiological processes such as differentiation proliferation and energy generation. Glycolysis changes also affect many diseases, including cancer, cardiovascular disease, and metabolic diseases.
Protons are released when cells release lactic acid produced by glycolysis. Therefore, extracellular acidification rate (ECAR) is an index of glycolysis. Creative Biogene uses Seahorse XF analyzer to measure proton external flow rate and mitochondrial oxygen consumption OCR (OCR) simultaneously to provide biological energy metabolism spectra of living cells. Unlike endpoint detection (e.g. lactic acid), Seahorse XF analyzer can detect changes in metabolic functions in real-time, helping to understand the earliest events of metabolic transformation.
General Strategy
ECAR is often used to study metabolic conditions such as glycolysis.
he final addition of 2-DG is a competitive inhibitor of hexokinase, which can block glycolysis, so the curve returns to the basic value after addition.
The basic value before the addition of glucose is the production of acid by non-fermentation, such as mitochondrial acidification caused by carbon dioxide produced by mitochondrial respiration. Glucose is then added, and the increased value represents glycolysis. After the addition of oligomycin, oxidative phosphorylation is inhibited, and the cells are forced to use lactic acid fermentation to supply energy, leading to increased acid production. The value at this time is called glycolysis capacity, and the difference between glycolysis and glycolysis is called glycolysis reserve. The final addition of 2-DG is a competitive inhibitor of hexokinase, which can block glycolysis, so the curve returns to the basic value after addition.
Related Services
Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.
Our Advantages
- Rich experience in determination and analysis of mitochondrial glycolysis
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